Journal: Bone Reports

Article Title: Intermittent fasting alleviates obesity-associated impairments in bone fracture healing: Exploring the role of gut microbiome

doi: 10.1016/j.bonr.2025.101876

Figure Lengend Snippet: Intermittent fasting reduces body weight of obese mice while body composition remains unchanged. A) Schematic of the study design and diet composition. The study time-line is shown in weeks, with fracture surgery designated as time point 0, B) X-Ray shows tibia fracture line (yellow arrow) and intramedullary fixation at 21-day post-fracture time point, C) Average daily food intake of mice on Ad libitum and IF (on feeding days) regimens was measured and normalized to mice body weight ( n = 6–8), D) Changes in body weight was measured weekly for 28 days prior to the fracture surgery (n = 6–8), E) DXA scan measurement of bone mineral density after four weeks of IF regimen was performed in mice on control and HFD diets (n = 6–8). F) DXA scan measurement of body fat percentage was performed after four weeks of IF in mice on control and HFD diets (n = 6–8). ** p < 0.01, **** p < 0.0001, ns = not significant, using an unpaired Student's t -test (C and D) and two-way ANOVA with Tukey's multiple comparisons test (E and F), black horizontal lines show mean and error bars show ±SEM.

Article Snippet: Following four weeks of IF and before the fracture surgery, DXA scans were performed on anesthetized mice to measure body composition (Hologic Faxitron Bioptics UltraFocus DXA ).

Techniques: Control

Journal: Bone Reports

Article Title: Intermittent fasting alleviates obesity-associated impairments in bone fracture healing: Exploring the role of gut microbiome

doi: 10.1016/j.bonr.2025.101876

Figure Lengend Snippet: Obesity causes dysbiosis in gut microbiome. A) Principal Coordinate Analysis (PCoA) plot of Generalized UniFrac analysis illustrates microbial dissimilarity in Control-Ad lib and HFD-IF groups at 21 days post-fracture (n = 5), each symbol represents one mouse. p = 0.01 based on Permutational ANOVA (PERMANOVA). B) Comparing the Shannon index of alpha-diversity between control- Ad lib and HFD-Ad lib mice (n = 5), C) Pie-charts illustrate the composition of the gut microbiome at phylum level and percentage of relative abundances for each phylum. n = 5 from each diet group is used to calculate average percentage abundances. D) Bar-chart illustrates percentage relative abundances of bacteria at family level, n = 5 from each diet group is used to calculate average percentage abundances. ns = not significant using Wilcoxon rank test (B).

Article Snippet: Following four weeks of IF and before the fracture surgery, DXA scans were performed on anesthetized mice to measure body composition (Hologic Faxitron Bioptics UltraFocus DXA ).

Techniques: Control, Bacteria

Journal: Bone Reports

Article Title: Intermittent fasting alleviates obesity-associated impairments in bone fracture healing: Exploring the role of gut microbiome

doi: 10.1016/j.bonr.2025.101876

Figure Lengend Snippet: Intermittent fasting alters gut microbiome composition in obese mice. A) PCoA plot of Generalized UniFrac analysis at the species level illustrates microbial dissimilarity in HFD-IF and HFD-Ad lib groups at 21 days post-fracture (n = 5), each dot represents one mouse, p = 0.045 based on Permutational ANOVA (PERMANOVA), B) The Shannon index of α-diversity was compared between two diet groups (n = 5), C) Pie Charts illustrate an average of major phyla percentage abundances in each cohort (n = 5). D) Relative abundance of six genera with significant difference between two groups is compared (n = 5), E) Histogram shows the Linear Discriminant Analysis (LDA) score of the effect size (LEfSe) identifying taxonomic differences between two cohorts. Horizontal bars represent the effect size of taxa or phylotype. Threshold of significance was set to LDA > 2 and p < 0.05. The name of the taxon level is abbreviated as f_family, g_genus, and s_species (n = 5). F) Histogram of LEfSe analysis LDA scores for differentially abundant microbial KEGG pathways predicted by PICRUSt, Threshold of significance was set to LDA > 2 and p < 0.05. (n = 5), * p < 0.05, *** p < 0.001, ns = not significant, using Wilcoxon rank test (B) and unpaired Student's t-test (D).

Article Snippet: Following four weeks of IF and before the fracture surgery, DXA scans were performed on anesthetized mice to measure body composition (Hologic Faxitron Bioptics UltraFocus DXA ).

Techniques: